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RStudio software version 1.1.456
Plant phenotype and phytoplasma titre in wild-type and Atcals7ko lines. a Representative images of healthy and CY-infected wild-type and Atcals7ko plants. Following CY infection, at 20 days after the inoculation access period (IAP), both plant lines showed yellowish small leaves. Leaves having emerged after phytoplasma inoculation were shorter, with thicker main veins and shorter petioles. b Fresh weight of rosettes was reduced in Atcals7ko plants by 35.3% (inset) as compared to wild-type plants. Following CY infection, rosette fresh weight, on average, was affected by a reduction by 16.5% (inset) in wild-type plants and by 56.4% (inset) in Atcals7ko mutants in comparison with their respective healthy controls. c , d The flower stalk was well developed in healthy wild-type but reduced in length (by 41%, inset) in Atcals7ko plants. Following CY infection, the stalk length was strongly reduced (by 88%, inset) in wild-type individuals and absent in the Atcals7ko mutants. e Phytoplasma titre [expressed as the number of CY-phytoplasma genome units (GUs) per mg of fresh leaf sample] strongly increased in mutant line in comparison to the wild type. In b and c, statistical analyses were performed using RStudio software Version 1.1.456 (RStudio Team  ). The normal distribution was checked with the Shapiro–Wilk test. Significant differences among the group means were determined with a two-way ANOVA and post-hoc comparisons between all groups were made with Tukey’s test with P < 0.05. In e , statistical analysis was performed using RStudio software version 1.1.456 (2009–2018 RStudio). The normal distribution was checked with Shapiro–Wilk test. Significant differences among the means were determined using the Kruskal–Wallis non-parametric test with P < 0.05. Error bars indicate standard error of the mean of 8 biological replicates for each condition
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Plant phenotype and phytoplasma titre in wild-type and Atcals7ko lines. a Representative images of healthy and CY-infected wild-type and Atcals7ko plants. Following CY infection, at 20 days after the inoculation access period (IAP), both plant lines showed yellowish small leaves. Leaves having emerged after phytoplasma inoculation were shorter, with thicker main veins and shorter petioles. b Fresh weight of rosettes was reduced in Atcals7ko plants by 35.3% (inset) as compared to wild-type plants. Following CY infection, rosette fresh weight, on average, was affected by a reduction by 16.5% (inset) in wild-type plants and by 56.4% (inset) in Atcals7ko mutants in comparison with their respective healthy controls. c , d The flower stalk was well developed in healthy wild-type but reduced in length (by 41%, inset) in Atcals7ko plants. Following CY infection, the stalk length was strongly reduced (by 88%, inset) in wild-type individuals and absent in the Atcals7ko mutants. e Phytoplasma titre [expressed as the number of CY-phytoplasma genome units (GUs) per mg of fresh leaf sample] strongly increased in mutant line in comparison to the wild type. In b and c, statistical analyses were performed using RStudio software Version 1.1.456 (RStudio Team  ). The normal distribution was checked with the Shapiro–Wilk test. Significant differences among the group means were determined with a two-way ANOVA and post-hoc comparisons between all groups were made with Tukey’s test with P < 0.05. In e , statistical analysis was performed using RStudio software version 1.1.456 (2009–2018 RStudio). The normal distribution was checked with Shapiro–Wilk test. Significant differences among the means were determined using the Kruskal–Wallis non-parametric test with P < 0.05. Error bars indicate standard error of the mean of 8 biological replicates for each condition

Journal: Planta

Article Title: Increased susceptibility to Chrysanthemum Yellows phytoplasma infection in Atcals7ko plants is accompanied by enhanced expression of carbohydrate transporters

doi: 10.1007/s00425-022-03954-8

Figure Lengend Snippet: Plant phenotype and phytoplasma titre in wild-type and Atcals7ko lines. a Representative images of healthy and CY-infected wild-type and Atcals7ko plants. Following CY infection, at 20 days after the inoculation access period (IAP), both plant lines showed yellowish small leaves. Leaves having emerged after phytoplasma inoculation were shorter, with thicker main veins and shorter petioles. b Fresh weight of rosettes was reduced in Atcals7ko plants by 35.3% (inset) as compared to wild-type plants. Following CY infection, rosette fresh weight, on average, was affected by a reduction by 16.5% (inset) in wild-type plants and by 56.4% (inset) in Atcals7ko mutants in comparison with their respective healthy controls. c , d The flower stalk was well developed in healthy wild-type but reduced in length (by 41%, inset) in Atcals7ko plants. Following CY infection, the stalk length was strongly reduced (by 88%, inset) in wild-type individuals and absent in the Atcals7ko mutants. e Phytoplasma titre [expressed as the number of CY-phytoplasma genome units (GUs) per mg of fresh leaf sample] strongly increased in mutant line in comparison to the wild type. In b and c, statistical analyses were performed using RStudio software Version 1.1.456 (RStudio Team ). The normal distribution was checked with the Shapiro–Wilk test. Significant differences among the group means were determined with a two-way ANOVA and post-hoc comparisons between all groups were made with Tukey’s test with P < 0.05. In e , statistical analysis was performed using RStudio software version 1.1.456 (2009–2018 RStudio). The normal distribution was checked with Shapiro–Wilk test. Significant differences among the means were determined using the Kruskal–Wallis non-parametric test with P < 0.05. Error bars indicate standard error of the mean of 8 biological replicates for each condition

Article Snippet: Statistical analyses were performed using RStudio software Version 1.1.456 (2009–2018 RStudio).

Techniques: Infection, Mutagenesis, Software